TaKaRa Taq Hot Start Version GMP grade DNA polymerase在嚴格的標準下制造，以確保高質量和一致性，符合GMP規定和指南的設施。GMP級別為熱啟動聚合酶帶來了更高水平的可信度和一致性，特別適用于苛刻的研究應用，包括多重PCR。 此GMP級試劑提供：
· 嚴格的規格
· 卓越的性能
· 可定制包裝
所有的Hot-start型Takara Taq聚合酶是抗Taq單克隆抗體和TaKaRa Taq 的混合制品，適用于Hot Start PCR。Hot Start PCR可以提高特異性，降低背景，可在室溫下配制反應液。因Hot Start PCR可防止非特異性擴增，高特異性是實驗成功的關鍵，因此是多重PCR的理想選擇。抗Taq單克隆抗體在PCR反應最初的DNA變性步驟已變性，因此在常規PCR反應條件下即可使用。
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Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration,is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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■ 保存
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-20℃。避免反復凍融。
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■ 特點
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Test Items
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Specifications
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Results
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Purity (microfluidic chip analysis)
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>98%
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Passed
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Ribonucleases
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No observable degradation
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Passed
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Endonucleases
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No observable degradation
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Passed
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Exonucleases
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No observable degradation
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Passed
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Nicking activities
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No observable relaxation of supercoiled DNA
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Passed
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Residual E. coli gDNA
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<10 copies/1.25 U
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Passed
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Hot-start activity
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Confirmed
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Passed
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PCR performance
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8-kb fragment was amplified
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Passed
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Sensitivity (λ DNA)
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0.5-kb fragment was amplified from 10 pg of λ DNA
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Passed
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Sensitivity (human gDNA)
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0.1-kb fragment was amplified from 30 pg of human
genomic DNA
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Passed
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■ 應用
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· Hot-start PCR
· Multiplex PCR
· qPCR
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