人iPS干細胞分化來的肝細胞
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Cellartis enhanced hiPS-HEP v2 kits 提供了可持續供應的、成熟的人誘導多能干細胞（hiPS）來源的肝臟細胞。在超過14天的檢測窗口期，hiPS-HEP細胞擁有功能性的藥物代謝機制，使其成為體外藥物研發、藥物代謝、和相關毒性研究的理想選擇；具備成熟、穩健的代謝特征，支持其作為代謝疾病模型。方便使用的試劑盒包括完全的培養基和組份，用于長期檢測。與其他肝臟細胞模型相比，培養的Cellartis hiPS-HEP細胞存活時間更長，進而擁有更長的檢測窗口用于慢性-毒性實驗和作為代謝疾病模型研究。
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■ 產品特點
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· Complete kit for thawing, plating, and culturing the provided cells
· Highly homogeneous population with consistent performance between batches and over time
· Cells are functional and stable for a 14-day assay window
· Cells show long-term, stable CYP450 activities
· Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin
· Cells secrete physiologically relevant levels of albumin and urea
· Cells demonstrate functional insulin and glucose regulation
· Cells show phase I and II enzyme activities
· Cells demonstrate LDL uptake
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■ 產品組成
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· 1 vial of ～1.2 × 107 cells
· Hepatocyte thawing medium
· Hepatocyte coating
· Hepatocyte plating medium
· Hepatocyte washing medium
· Hepatocyte long-term maintenance medium
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■ 產品應用
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· Toxicity testing, including chronic toxicity assays
· Drug discovery and development
· Metabolic studies
· Metabolic disease modeling
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Code No.
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Product
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Size
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Y10133
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Cellartis® Enhanced hiPS-HEP (from ChiPSC12) v2 Kitt
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1 Kit
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Y10134
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Cellartis® Enhanced hiPS-HEP (from ChiPSC18) v2 Kit
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1 Kit
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Y10135
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Cellartis® Enhanced hiPS-HEP (from ChiPSC22) v2 Kit
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1 Kit
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■ 產品詳情
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http://www.clontech.com/CN/Products/Stem_Cell_Research/Cells/Human_Stem_Cell_Derived
_Hepatocytes?sitex=10022:22372:US
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Cellartis 人iPS細胞來源的肝臟細胞可以作為長期的、可靠的肝臟疾病模型。
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Cellartis human induced pluripotent stem (iPS) cell-derived hepatocytes are a long-lasting, reliable liver disease model. Ideal for long-term studies, these mature, functional, pure hepatocytes allow you to generate consistent results with low batch-to-batch variability. Cellartis iPSC-derived hepatocytes can be used for extended culture significantly longer than human primary hepatocytes—allowing you to get more data from your chronic toxicity studies.
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Cellartis enhanced hiPS-HEP cells可以穩定表達CYP450 活性超過21天。
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CYP450 activity is stable in enhanced hiPS-HEP cells over 21 days. LC/MS was used to analyze CYP450 activity in cultured enhanced hiPS-HEP cells previously derived from the hiPS cell lines ChiPSC12, ChiPSC18, and ChiPSC22 (abbreviated as C12, C18, and C22). CYP3A (Panel A), CYP2C9 (Panel B), CYP1A (Panel C), and CYP2C19 (Panel D) activities in enhanced hiPS-HEP cells are stable over an extended culture time. Cryopreserved human primary hepatocytes (hphep), which are functional in culture for a significantly shorter time than enhanced hiPS-HEP cells, were thawed and cultured for 20 hours, and their data are shown as the black bar in each panel.
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Cellartis enhanced hiPS-HEP細胞在20天的培養中表達白蛋白（Albumin）。
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Albumin is present in enhanced hiPS-HEP cells through 20 days in culture. Panel A. Representative images of enhanced hiPS-HEP cells from human iPS cell line ChiPSC18 (C18) taken 12 days after thawing (right), compared to cryopreserved human primary hepatocytes (hphep) taken 24 hr after thawing (left). Cells were stained for albumin and DAPI. Panel B. Albumin secretion as measured by ELISA; n=2 for enhanced hiPS-HEP cells, with the exception of C18 at 20 days (n=1), and n=3 donors for hphep.
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（以上圖片均來源于Takara Bio USA, Inc.）
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參考文獻：
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1. Heins et al. Stem Cells 2004; 22: 367-376.United States National Stem Cell Bank; http://www.nationalstemcellbank.org.
2. Mantel N et al. Potential markers of attenuation of YF virus after infection of stem cell-derived human hepatocytes with wild-type Asibi or live-attenuated YF17D virus.Supplement to The American Journal of tropical Medicine and Hygiene, Volume 83, November 2010, Number 5, abstract 12.
3. Yildirimman R et al. Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitrohazard assessment of chemical carcinogenicity. Toxicol. Sci. 2011 Dec; 124(2): 278-90.
4. Ulvestad M et al. Drug metabolizing enzyme and transporter protein profi les of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem Pharmacol. 2013 Sep 1; 86(5):691-702.
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